César Milstein

Report
450
0
Vote World Rank
117
Vote Argentina Rank
2

Country: Argentina

About César Milstein
César Milstein (8 October 1927 – 24 March 2002) was an Argentine biochemist in the field of antibody research. Milstein shared the Nobel Prize in Physiology or Medicine in 1984 with Niels K. Jerne and Georges Köhler.Biography Milstein was born in Bahía Blanca, Argentina. He graduated from the University of Buenos Aires and obtained a PhD under Professor Stoppani (Professor of Biochemistry) in the Medical School on kinetic studies with the enzyme aldehyde dehydrogenase. In 1958, funded by the British Council, he joined the Biochemistry Department at the University of Cambridge to work for a PhD under Malcolm Dixon on the mechanism of metal activation of the enzyme phosphoglucomutase. During this work he collaborated with Frederick Sanger whose group he joined with a short-term Medical Research Council appointment. Career The major part of Milstein's research career was devoted to studying the structure of antibodies and the mechanism by which antibody diversity is generated. It was as part of this quest that in 1975 he, together with Georges Köhler (a postdoctoral fellow in his laboratory), developed the hybridoma technique for the production of monoclonal antibodies—a discovery recognised by the award of the 1984 Nobel Prize for Physiology or Medicine. This discovery led to an enormous expansion in the exploitation of antibodies in science and medicine. The monoclonal antibody experiment was the control experiment to one suggested by Prof.Pieczenik, which is known as the Cotton-Milstein experiment. This was the fusion of two known myeloma cells with defined constant and variable regions to identify at which level the recombination of coding occurred i.e. nucleotide or protein. This experiment is a variation of the Zinder-Edelman experiment where they dissociated immunoglobulin heavy and light chains produced against f1 and f2 bacteriophage. They then created heteroduplexes of these light and heavy chains to identify antibody binding specificity. Heteroduplexes do not bind properly. This has enormous consequences on interpreting the combinatorial phage libraries being commercially produced presently.

Best Supporters

How can you earn points?
1 Vote 1 Point [You must be logged in]
1 Referrer* 10 Points
*Call your friends to become a fan of César Milstein with your referrer link and earn 10 points

César Milstein Most Popular Quotes

Add Quote

Argentina Scientists & Academicians Ranking

Show All
Rank Name Total
1. René Favaloro 2044
2. César Milstein 450
3. Bernardo Houssay 97
4. Luis Leloir 86

Scientists & Academicians Ranking

Show All
Rank Name Total
113. Morteza Motahhari 527
114. Michio Kaku 515
115. George Emil Palade 473
116. Ejup Ganic 460
117. César Milstein 450
118. Benjamin Franklin 445
119. Sergey Korolyov 442
120. G. Madhavan Nair 420
121. Nassim Nicholas 411

César Milstein Photos

Today Overall Show All

César Milstein Videos

Today Overall Show All

Best Countries

Today Overall
# Name Today Total
1 Turkey 18730 7958685
2 Romania 5770 510948
3 India 3956 3684228
4 Brazil 3745 4688462
5 South Korea 3258 1707318
6 Portugal 1641 276670
7 Iran 1013 4155365
8 Russia 934 1319285
9 Germany 745 72424
10 United States of America 573 1419638
11 Syria 498 1387991
12 United Kingdom 486 886087
13 Macedonia 453 950139
14 Serbia 446 209550
15 Poland 335 403632
16 Ukraine 271 227944
17 Saudi Arabia 263 768999
18 Israel 229 66694
19 Bulgaria 220 322908
20 Jordan 212 517287
21 France 138 83961
22 Palestine 120 151461
23 Lebanon 97 540869
24 Bosnia and Herzegovina 58 359783
25 Greece 48 416114
26 Pakistan 41 274654
27 Sri Lanka 39 8596
28 Norway 17 14656
29 Iraq 15 850045
30 Afghanistan 1 5591482
31 Albania 0 2207
32 Algeria 0 94755
33 Andorra 0 0
34 Angola 0 0
35 Antigua and Barbuda 0 0
36 Argentina 0 59184
37 Armenia 0 1095
38 Australia 0 714
39 Austria 0 377
40 Azerbaijan 0 14798
41 Bahamas 0 0
42 Bahrain 0 16643
43 Bangladesh 0 3923
44 Barbados 0 0
45 Belarus 0 2612
46 Belgium 0 8603
47 Belize 0 0
48 Benin 0 0
49 Bermuda 0 0
50 Bhutan 0 0
51 Bolivia 0 5434
52 Greenland 0 0
53 Grenada 0 0
54 Guatemala 0 0
55 Guinea 0 4
56 Guinea-Bissau 0 0
57 Guyana 0 0
58 Haiti 0 0
59 Honduras 0 0
60 Hong Kong 0 201
61 Hungary 0 2675
62 Iceland 0 230
63 Botswana 0 0
64 Brunei Darussalem 0 0
65 Gabon 0 0
66 The Gambia 0 0
67 Georgia 0 91
68 Burkina Faso 0 0
69 Burundi 0 0
70 Cambodia 0 127
71 Cameroon 0 5
72 Canada 0 23166
73 Cape Verde 0 65
74 Cayman Islands 0 0
75 Central African Republic 0 0
76 Chad 0 0
77 Chile 0 10649
78 People's Republic of China 0 1001
79 Republic of China-Taiwan 0 33
80 Comoros 0 1
81 Democratic Republic of the Congo 0 0
82 Republic of the Congo 0 9
83 Cook Islands 0 0
84 Costa Rica 0 0
85 Cote d'Ivoire 0 20
86 Croatia 0 32005
87 Cuba 0 29
88 Cyprus 0 1903
89 Czech Republic 0 19817
90 Denmark 0 352
91 Djibouti 0 0
92 Dominica 0 0
93 Dominican Republic 0 0
94 Ecuador 0 4
95 Egypt 0 958935
96 El Salvador 0 0
97 Equatorial Guinea 0 0
98 Eritrea 0 0
99 Estonia 0 0
100 Ethiopia 0 13228