César Milstein

Report
450
0
Vote World Rank
116
Vote Argentina Rank
2

Country: Argentina

About César Milstein
César Milstein (8 October 1927 – 24 March 2002) was an Argentine biochemist in the field of antibody research. Milstein shared the Nobel Prize in Physiology or Medicine in 1984 with Niels K. Jerne and Georges Köhler.Biography Milstein was born in Bahía Blanca, Argentina. He graduated from the University of Buenos Aires and obtained a PhD under Professor Stoppani (Professor of Biochemistry) in the Medical School on kinetic studies with the enzyme aldehyde dehydrogenase. In 1958, funded by the British Council, he joined the Biochemistry Department at the University of Cambridge to work for a PhD under Malcolm Dixon on the mechanism of metal activation of the enzyme phosphoglucomutase. During this work he collaborated with Frederick Sanger whose group he joined with a short-term Medical Research Council appointment. Career The major part of Milstein's research career was devoted to studying the structure of antibodies and the mechanism by which antibody diversity is generated. It was as part of this quest that in 1975 he, together with Georges Köhler (a postdoctoral fellow in his laboratory), developed the hybridoma technique for the production of monoclonal antibodies—a discovery recognised by the award of the 1984 Nobel Prize for Physiology or Medicine. This discovery led to an enormous expansion in the exploitation of antibodies in science and medicine. The monoclonal antibody experiment was the control experiment to one suggested by Prof.Pieczenik, which is known as the Cotton-Milstein experiment. This was the fusion of two known myeloma cells with defined constant and variable regions to identify at which level the recombination of coding occurred i.e. nucleotide or protein. This experiment is a variation of the Zinder-Edelman experiment where they dissociated immunoglobulin heavy and light chains produced against f1 and f2 bacteriophage. They then created heteroduplexes of these light and heavy chains to identify antibody binding specificity. Heteroduplexes do not bind properly. This has enormous consequences on interpreting the combinatorial phage libraries being commercially produced presently.

Best Supporters

How can you earn points?
1 Vote 1 Point [You must be logged in]
1 Referrer* 10 Points
*Call your friends to become a fan of César Milstein with your referrer link and earn 10 points

César Milstein Most Popular Quotes

Add Quote

Argentina Scientists & Academicians Ranking

Show All
Rank Name Total
1. René Favaloro 2044
2. César Milstein 450
3. Bernardo Houssay 97
4. Luis Leloir 86

Scientists & Academicians Ranking

Show All
Rank Name Total
112. Michio Kaku 515
113. Anghel Saligny 461
114. Victor Babes 461
115. Ejup Ganic 460
116. César Milstein 450
117. Benjamin Franklin 445
118. Sergey Korolyov 442
119. G. Madhavan Nair 420
120. Nassim Nicholas 411

César Milstein Photos

Today Overall Show All

César Milstein Videos

Today Overall Show All

Best Countries

Today Overall
# Name Today Total
1 Turkey 1376 7584745
2 Brazil 281 4598892
3 Russia 171 1276881
4 India 114 3627388
5 South Korea 103 1643477
6 Iran 63 4134128
7 Ukraine 50 221721
8 United States of America 37 1403559
9 Syria 29 1378097
10 United Kingdom 26 875743
11 Portugal 25 250196
12 Macedonia 22 942564
13 Poland 22 389448
14 Serbia 21 200850
15 Saudi Arabia 14 762787
16 Israel 11 62174
17 Jordan 11 513431
18 Palestine 10 148468
19 Afghanistan 7 5590609
20 France 3 82126
21 Germany 3 66604
22 Bulgaria 3 320478
23 Sri Lanka 3 7737
24 Norway 2 14087
25 Iraq 2 849726
26 Bosnia and Herzegovina 2 358575
27 Belarus 2 2391
28 Sweden 2 22030
29 Lebanon 1 539508
30 Lesotho 0 0
31 Liberia 0 3
32 Libya 0 143
33 Liechtenstein 0 0
34 Lithuania 0 35
35 Luxembourg 0 0
36 Macau 0 0
37 Kazakhstan 0 228
38 Kenya 0 4
39 Kiribati 0 0
40 North Korea 0 0
41 Italy 0 62027
42 Jamaica 0 8060
43 Japan 0 8819
44 Madagascar 0 0
45 Malawi 0 0
46 Malaysia 0 11330
47 Maldives 0 3
48 Mali 0 0
49 Malta 0 311
50 Mauritania 0 0
51 Mauritius 0 0
52 Mexico 0 37798
53 Moldova 0 11001
54 Monaco 0 31
55 Mongolia 0 2
56 Montenegro 0 96
57 Morocco 0 347002
58 Mozambique 0 0
59 Myanmar 0 40
60 Namibia 0 0
61 Nauru 0 0
62 Nepal 0 0
63 Netherlands 0 282
64 New Zealand 0 12332
65 Nicaragua 0 0
66 Niger 0 0
67 Nigeria 0 10
68 Northern Cyprus 0 374
69 Oman 0 4
70 Pakistan 0 274065
71 Senegal 0 0
72 Sudan 0 147
73 Suriname 0 0
74 Swaziland 0 0
75 Seychelles 0 0
76 Sierra Leone 0 0
77 Singapore 0 1
78 Slovakia 0 11880
79 Slovenia 0 57
80 Solomon Islands 0 0
81 Somalia 0 32
82 Somaliland 0 0
83 South Africa 0 267
84 Spain 0 82248
85 Panama 0 1
86 Papua New Guinea 0 0
87 Paraguay 0 13
88 Peru 0 1632
89 Philippines 0 617
90 Kosovo 0 2950
91 Kuwait 0 63815
92 Kyrgyzstan 0 93
93 Laos 0 0
94 Latvia 0 0
95 Puerto Rico 0 1384
96 Qatar 0 0
97 Romania 0 442465
98 Rwanda 0 0
99 Saint Kitts and Nevis 0 0
100 Saint Lucia 0 0