César Milstein

Report
450
0
Vote World Rank
117
Vote Argentina Rank
2

Country: Argentina

About César Milstein
César Milstein (8 October 1927 – 24 March 2002) was an Argentine biochemist in the field of antibody research. Milstein shared the Nobel Prize in Physiology or Medicine in 1984 with Niels K. Jerne and Georges Köhler.Biography Milstein was born in Bahía Blanca, Argentina. He graduated from the University of Buenos Aires and obtained a PhD under Professor Stoppani (Professor of Biochemistry) in the Medical School on kinetic studies with the enzyme aldehyde dehydrogenase. In 1958, funded by the British Council, he joined the Biochemistry Department at the University of Cambridge to work for a PhD under Malcolm Dixon on the mechanism of metal activation of the enzyme phosphoglucomutase. During this work he collaborated with Frederick Sanger whose group he joined with a short-term Medical Research Council appointment. Career The major part of Milstein's research career was devoted to studying the structure of antibodies and the mechanism by which antibody diversity is generated. It was as part of this quest that in 1975 he, together with Georges Köhler (a postdoctoral fellow in his laboratory), developed the hybridoma technique for the production of monoclonal antibodies—a discovery recognised by the award of the 1984 Nobel Prize for Physiology or Medicine. This discovery led to an enormous expansion in the exploitation of antibodies in science and medicine. The monoclonal antibody experiment was the control experiment to one suggested by Prof.Pieczenik, which is known as the Cotton-Milstein experiment. This was the fusion of two known myeloma cells with defined constant and variable regions to identify at which level the recombination of coding occurred i.e. nucleotide or protein. This experiment is a variation of the Zinder-Edelman experiment where they dissociated immunoglobulin heavy and light chains produced against f1 and f2 bacteriophage. They then created heteroduplexes of these light and heavy chains to identify antibody binding specificity. Heteroduplexes do not bind properly. This has enormous consequences on interpreting the combinatorial phage libraries being commercially produced presently.

Best Supporters

How can you earn points?
1 Vote 1 Point [You must be logged in]
1 Referrer* 10 Points
*Call your friends to become a fan of César Milstein with your referrer link and earn 10 points

César Milstein Most Popular Quotes

Add Quote

Argentina Scientists & Academicians Ranking

Show All
Rank Name Total
1. René Favaloro 2044
2. César Milstein 450
3. Bernardo Houssay 97
4. Luis Leloir 87

Scientists & Academicians Ranking

Show All
Rank Name Total
113. Jan Amos Komenský 555
114. Morteza Motahhari 527
115. Michio Kaku 515
116. Ejup Ganic 460
117. César Milstein 450
118. Benjamin Franklin 445
119. Sergey Korolyov 442
120. G. Madhavan Nair 420
121. Daniel Fahrenheit 414

César Milstein Photos

Today Overall Show All

César Milstein Videos

Today Overall Show All

Best Countries

Today Overall
# Name Today Total
1 Turkey 203272 8143227
2 Romania 53173 558351
3 Brazil 51467 4736184
4 South Korea 42496 1746556
5 India 41823 3722095
6 Portugal 23485 298514
7 Russia 16924 1335275
8 Iran 14064 4168416
9 United Kingdom 7174 892775
10 Macedonia 6818 956504
11 Germany 6810 78489
12 United States of America 6796 1425861
13 Syria 6579 1394072
14 Serbia 5893 214997
15 Poland 3738 407035
16 Ukraine 3722 231395
17 Israel 3037 69502
18 Lebanon 2440 543212
19 Jordan 2256 519331
20 Saudi Arabia 2162 770898
21 France 1751 85574
22 Bulgaria 1722 324410
23 Palestine 1392 152733
24 Bosnia and Herzegovina 827 360552
25 Norway 704 15343
26 Sri Lanka 631 9188
27 Slovakia 461 12341
28 Pakistan 349 274962
29 Iraq 203 850233
30 Italy 199 62300
31 Greece 174 416240
32 Afghanistan 46 5591527
33 Estonia 43 43
34 Latvia 42 42
35 Czech Republic 36 19853
36 Spain 22 83258
37 Argentina 21 59205
38 Lithuania 17 52
39 Egypt 13 958948
40 Hungary 13 2688
41 Monaco 10 41
42 Canada 9 23175
43 Indonesia 8 43756
44 Uruguay 7 513
45 Belarus 5 2617
46 Malaysia 4 11334
47 Faroe Islands 2 3014
48 Tunisia 2 40346
49 Turkmenistan 2 6
50 Swaziland 2 2
51 Slovenia 1 58
52 Switzerland 1 603122
53 Ethiopia 1 13229
54 Bahrain 1 16644
55 Mexico 1 37801
56 Georgia 1 92
57 Luxembourg 0 0
58 Macau 0 0
59 Lesotho 0 0
60 Liberia 0 3
61 Libya 0 143
62 Liechtenstein 0 0
63 Moldova 0 11059
64 Madagascar 0 0
65 Malawi 0 0
66 Maldives 0 3
67 Mali 0 0
68 Malta 0 311
69 Mauritania 0 0
70 Mauritius 0 0
71 Ireland 0 2249
72 Jamaica 0 8060
73 Japan 0 8819
74 Kazakhstan 0 228
75 Kenya 0 4
76 Kiribati 0 0
77 North Korea 0 0
78 Kosovo 0 2950
79 Kuwait 0 63815
80 Kyrgyzstan 0 93
81 Laos 0 0
82 Mongolia 0 2
83 Montenegro 0 96
84 Morocco 0 347003
85 Mozambique 0 0
86 Myanmar 0 40
87 Namibia 0 0
88 Nauru 0 0
89 Nepal 0 0
90 Netherlands 0 282
91 New Zealand 0 12332
92 Nicaragua 0 0
93 Niger 0 0
94 Nigeria 0 10
95 Northern Cyprus 0 374
96 Oman 0 4
97 Panama 0 1
98 Papua New Guinea 0 0
99 Paraguay 0 13
100 Peru 0 1632