César Milstein

Report
450
0
Vote World Rank
116
Vote Argentina Rank
2

Country: Argentina

About César Milstein
César Milstein (8 October 1927 – 24 March 2002) was an Argentine biochemist in the field of antibody research. Milstein shared the Nobel Prize in Physiology or Medicine in 1984 with Niels K. Jerne and Georges Köhler.Biography Milstein was born in Bahía Blanca, Argentina. He graduated from the University of Buenos Aires and obtained a PhD under Professor Stoppani (Professor of Biochemistry) in the Medical School on kinetic studies with the enzyme aldehyde dehydrogenase. In 1958, funded by the British Council, he joined the Biochemistry Department at the University of Cambridge to work for a PhD under Malcolm Dixon on the mechanism of metal activation of the enzyme phosphoglucomutase. During this work he collaborated with Frederick Sanger whose group he joined with a short-term Medical Research Council appointment. Career The major part of Milstein's research career was devoted to studying the structure of antibodies and the mechanism by which antibody diversity is generated. It was as part of this quest that in 1975 he, together with Georges Köhler (a postdoctoral fellow in his laboratory), developed the hybridoma technique for the production of monoclonal antibodies—a discovery recognised by the award of the 1984 Nobel Prize for Physiology or Medicine. This discovery led to an enormous expansion in the exploitation of antibodies in science and medicine. The monoclonal antibody experiment was the control experiment to one suggested by Prof.Pieczenik, which is known as the Cotton-Milstein experiment. This was the fusion of two known myeloma cells with defined constant and variable regions to identify at which level the recombination of coding occurred i.e. nucleotide or protein. This experiment is a variation of the Zinder-Edelman experiment where they dissociated immunoglobulin heavy and light chains produced against f1 and f2 bacteriophage. They then created heteroduplexes of these light and heavy chains to identify antibody binding specificity. Heteroduplexes do not bind properly. This has enormous consequences on interpreting the combinatorial phage libraries being commercially produced presently.

Best Supporters

How can you earn points?
1 Vote 1 Point [You must be logged in]
1 Referrer* 10 Points
*Call your friends to become a fan of César Milstein with your referrer link and earn 10 points

César Milstein Most Popular Quotes

Add Quote

Argentina Scientists & Academicians Ranking

Show All
Rank Name Total
1. René Favaloro 2044
2. César Milstein 450
3. Bernardo Houssay 97
4. Luis Leloir 86

Scientists & Academicians Ranking

Show All
Rank Name Total
112. Anghel Saligny 539
113. Morteza Motahhari 527
114. Michio Kaku 515
115. Ejup Ganic 460
116. César Milstein 450
117. Benjamin Franklin 445
118. Sergey Korolyov 442
119. George Emil Palade 439
120. G. Madhavan Nair 420

César Milstein Photos

Today Overall Show All

César Milstein Videos

Today Overall Show All

Best Countries

Today Overall
# Name Today Total
1 Turkey 527 7867664
2 South Korea 191 1691306
3 Portugal 142 269192
4 Brazil 142 4669933
5 Romania 86 491616
6 India 70 3670320
7 Russia 54 1314337
8 United States of America 34 1416979
9 Iran 33 4150291
10 Poland 30 401314
11 Germany 22 69615
12 Syria 17 1385819
13 Serbia 14 207293
14 Saudi Arabia 13 767841
15 United Kingdom 12 883811
16 Macedonia 11 947958
17 Ukraine 9 226873
18 Israel 7 65608
19 Palestine 7 150875
20 Bulgaria 5 321972
21 Lebanon 4 540497
22 Jordan 3 516437
23 Greece 3 415925
24 Sri Lanka 3 8407
25 Bosnia and Herzegovina 2 359522
26 Pakistan 2 274448
27 Norway 2 14567
28 Iraq 1 849967
29 Ireland 0 2249
30 Oman 0 4
31 Kosovo 0 2950
32 Kuwait 0 63815
33 Kyrgyzstan 0 93
34 Laos 0 0
35 Latvia 0 0
36 Puerto Rico 0 1384
37 Qatar 0 0
38 Panama 0 1
39 Papua New Guinea 0 0
40 Paraguay 0 13
41 Peru 0 1632
42 Philippines 0 617
43 Seychelles 0 0
44 Sierra Leone 0 0
45 Singapore 0 1
46 Slovakia 0 11880
47 Slovenia 0 57
48 Solomon Islands 0 0
49 Somalia 0 32
50 Somaliland 0 0
51 South Africa 0 267
52 Spain 0 83236
53 Rwanda 0 0
54 Saint Kitts and Nevis 0 0
55 Saint Lucia 0 0
56 Samoa 0 0
57 San Marino 0 0
58 Sao Tome and Principe 0 0
59 Kazakhstan 0 228
60 Kenya 0 4
61 Kiribati 0 0
62 North Korea 0 0
63 Italy 0 62100
64 Jamaica 0 8060
65 Japan 0 8819
66 Lesotho 0 0
67 Liberia 0 3
68 Libya 0 143
69 Liechtenstein 0 0
70 Lithuania 0 35
71 Luxembourg 0 0
72 Macau 0 0
73 Madagascar 0 0
74 Malawi 0 0
75 Malaysia 0 11330
76 Maldives 0 3
77 Mali 0 0
78 Malta 0 311
79 Mauritania 0 0
80 Mauritius 0 0
81 Mexico 0 37800
82 Moldova 0 11059
83 Monaco 0 31
84 Mongolia 0 2
85 Montenegro 0 96
86 Morocco 0 347002
87 Mozambique 0 0
88 Myanmar 0 40
89 Namibia 0 0
90 Nauru 0 0
91 Nepal 0 0
92 Netherlands 0 282
93 New Zealand 0 12332
94 Nicaragua 0 0
95 Niger 0 0
96 Nigeria 0 10
97 Northern Cyprus 0 374
98 Botswana 0 0
99 Brunei Darussalem 0 0
100 Ghana 0 3